Association analysis of HLA-DRB1*1501-DRB5*01 haplotype in multiple sclerosis population of Khuzestan Province

  • Hamid Galehdari Department of Genetics, Faculty of Sciences, Shahid Chamran University of Ahvaz, Iran
Keywords: Multiple Sclerosis, HLA-DRB5*01, HLA-DRB1*1501, PCR-SSP, Iran

Abstract

ABSTRACTBackground: Multiple sclerosis is an inflammatory, demyelinating disease of the central nervous system. Both genetic and environmental factors are involved in the MS pathogenesis. The human leukocyte antigen (HLA-II) alleles HLA-DRB1*1501, -DRB5*01, -DQA1*0102, -DQB1*0602 may have an important genetic effect. However, this is controversial in different population studies. Since, there are no data with respect to the association of HLA-DRB1*1501-DRB5*01 with MS in Khuzestan, the aim of this study was to investigate the correlation of this haplotype with MS in this population.Methods: The study focused on DRB5*01-DRB1*1501 haplotype association in 200 MS patients and 200 healthy individuals. HLA-typing was performed by polymerase chain reaction amplification with sequence-specific-primers (PCR-SSP) method. The statistical analyses were performed by SPSS software.Results: We found no association between DRB5*01+-DRB1*1501+ with MS (p=0.156). Distribution of DRB1*1501+-DRB5*01- (carrying DRB1*1501+ but not DRB5*01-) and DRB1*1501--DRB5*01- haplotypes were statistically different between patients and controls "(29.73% vs. 11.81%, p=0.000) and (42.16% vs. 68.50%, p=0.000)", respectively. However, DRB1*1501--DRB5*01+ showed no association with the disease (15.135% vs. 11.81%, p=0.403). HLA-DRB1*1501--DRB5*01+ was significantly more frequent among female MS patients (16.19% vs. 6.12%, p=0.019) and Fars group (17.11% vs. 5.79%, p=0.027).  HLA-DRB1*1501+-DRB5*01- haplotype was positively associated with EDSS steps 5 to 10 (62.5% vs. 25.76%, p=0.026). Furthermore, No meaningful correlation was observed among these haplotypes with sex, ethnicity, MS course and EDSS.Conclusions: Our results indicate that DRB1*1501+-DRB5*01- and DRB1*1501--DRB5*01- haplotypes may have correlation with MS risk. Also, our findings suggest that HLA-DRB1*1501--DRB5*01+ is involved in MS susceptibility among females and Persians. DRB1*1501+-DRB5*01- genotype frequency may play a role in MS developing.

References

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Published
2018-05-28
How to Cite
1.
Galehdari H. Association analysis of HLA-DRB1*1501-DRB5*01 haplotype in multiple sclerosis population of Khuzestan Province. IJNL. 17(3).
Section
Original Article(s)