Association of human leukocyte antigen-DRB haplotype in multiple sclerosis population of Khuzestan, Iran

  • Nooshin Delfan Department of Genetics, School of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
  • Hamid Galehdari Department of Genetics, School of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
  • Mohammad Shafiei Department of Genetics, School of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
  • Farideh Ghanbari-Mardasi Department of Genetics, School of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
  • Tahereh Latifi Department of Genetics, School of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
  • Nastaran Majdinasab Multiple Sclerosis Society, School of Rehabilitation, Ahvaz Jondishapour University of Medical Sciences, Ahvaz, Iran
  • Tahereh Seifi Department of Genetics, School of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Keywords: Multiple Sclerosis, Human Leukocyte Antigen-DRB5, Human Leukocyte Antigen-DRB1*1501, Polymerase Chain Reaction, Iran


Background: One of the demyelinating and inflammatory diseases of the central nervous system (CNS) is multiple sclerosis (MS). Though pathogenesis of MS is still unknown, both genetic and environmental factors are involved. The human leukocyte antigen (HLA) class-II alleles including HLA-DRB5*01, DQB1*0602, DRB1*1501, and DQA1*0102 may have remarkable effect in MS risk although it is controversial in studies. As there is no data with respect to the HLA-DRB1*1501-DRB5*01 correlation with MS in Khuzestan Province, Iran, the goal of the survey was to investigate the association of this haplotype with MS in this population.

Methods: The study focused on DRB5*01-DRB1*1501 haplotype association with MS in 200 patients and 200 healthy individuals. Typing of HLA was carried out by polymerase chain reaction (PCR) amplification with sequence-specific primers (SSP) method. SPSS software was used for the statistical analyses.

Results: No association between DRB5*01+-DRB1*1501+ and MS was found (P = 0.156). Distribution of DRB1*1501+-DRB5*01- (carrying DRB1*1501+ but not DRB5*01-) and DRB1*1501--DRB5*01- haplotypes was statistically different between patients and controls (29.73% vs. 11.81%, P < 0.001) and (42.16% vs. 68.50%, P < 0.001), respectively. However, DRB1*1501--DRB5*01+ revealed no association with MS (15.13% vs. 11.81%, P = 0.403). HLA-DRB1*1501--DRB5*01+ was significantly more frequent among female patients with MS (16.19% vs. 6.12%, P = 0.019) and Persian group (17.11% vs. 5.79%, P = 0.027). Positive correlation of HLA-DRB1*1501+-DRB5*01- haplotype with the expanded disability status scale (EDSS) steps from 5 to 10 was observed (62.50% vs. 25.76%, P = 0.026). Moreover, no meaningful association was shown among the haplotypes with EDSS, course of MS, ethnicity, and gender.

Conclusion: Our findings suggest that DRB1*1501+-DRB5*01- and DRB1*1501--DRB5*01- haplotypes may have positive association with MS risk. Also, this survey indicates that HLA-DRB1*1501--DRB5*01+ is involved in susceptibility of the disease among women and Persians. DRB1*1501+-DRB5*01- genotype frequency may have a key role in MS developing.


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How to Cite
Delfan N, Galehdari H, Shafiei M, Ghanbari-Mardasi F, Latifi T, Majdinasab N, Seifi T. Association of human leukocyte antigen-DRB haplotype in multiple sclerosis population of Khuzestan, Iran. Iran J Neurol. 17(4):154-160.
Original Article(s)