Association of 5-lipoxygenase activating protein gene polymorphism and stroke: A study from north east of Iran

  • Marjan Erfani Department of Neurology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  • Ariane Sadr-Nabavi Department of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  • Reza Jafarzadeh-Esfehani Department of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  • Mohammad Shariati Department of Neurology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  • Leila Ghanbari-Garekani Department of Neurology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  • Samaneh Vojdani-Chahchaheh Department of Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Payam Sasannejad Department of Neurology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Keywords: Stroke, Polymorphism, Genetic, Polymerase Chain Reaction, Restriction Fragment Length

Abstract

Background: Stroke is a multifactorial disorder and a major cause of morbidity and mortality around the world. There are growing numbers of candidate gene pathways which are thought to be associated with stroke. Genes involved in lipid metabolism are important issues in stroke studies. Studying different polymorphisms in these genes are becoming an interest for researchers. 5-lipoxygenase activating protein (ALOX5AP) is one of these genes. Different studies have provided different relations between ALOX5AP promoter polymorphism (rs17222919) and stroke. In the present study, we have evaluated this gene polymorphism in a population in north east of Iran.

Methods: This case-control study took place in Ghaem Hospital, Mashhad, Iran. Patients with computed tomography (CT) or magnetic resonance imaging (MRI) confirmation for ischemic stroke were enrolled in this study and considered as case group. Healthy persons without ischemic stroke were control group. During
1-year period of this study, ALOX5AP gene polymorphism in 200 healthy patients (control group) as well as 228 patients with stroke (case group) was evaluated by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).

Results: All of 428 persons (228 cases and 200 healthy controls) enrolled in this study. The genotype and allele frequency was significantly different between both groups (P = 0.001 and P = 0.003, respectively). A total number of 54 patients had G allele in case group in contrast to 27 ones in control group. Also, 174 patients in case group had T allele and 173 persons had this allele in control group. In compression of TT genotype, the risk of developing stroke in GG and TG genotypes increased by 3.998 and 1.643, respectively. Also the risk of ischemic stroke with G allele would increase by 2.128.

Conclusion: According to our results, ALOX5AP promoter polymorphism (rs17222919) is related to increased ischemic stroke in Iranian population.

References

1. Delbari A, Salman RR, Tabatabaei SS, Rahgozar M, Lokk J. Stroke epidemiology and one-month fatality among an urban population in Iran. Int J Stroke 2011; 6(3): 195-200.
2. Janghorbani M, Hamzehee-Moghadam A, Kachoiee H. Epidemiology of non-fatal stroke in South-Eastern Iran. Iran J Med Sci 1996; 21(3-4): 135-9.
3. Ahangar AA, Ashraf Vaghefi SB, Ramaezani M. Epidemiological evaluation of stroke in Babol, northern Iran (2001-2003). Eur Neurol 2005; 54(2): 93-7.
4. Azarpazhooh MR, Etemadi MM, Donnan GA, Mokhber N, Majdi MR, Ghayour-Mobarhan M, et al. Excessive incidence of stroke in Iran: Evidence from the mashhad stroke incidence study (msis), a population-based study of stroke in the middle east. Stroke 2010; 41(1): e3-e10.
5. Feigin VL, Norrving B, Mensah GA. Global burden of stroke. Circ Res 2017; 120(3): 439-48.
6. Shahjouei S, Bavarsad-Shahripour R, Assarzadegan F, Rikhtegar R, Mehrpour M, Zamani B, et al. Acute management of stroke in Iran: Obstacles and solutions. Iran J Neurol 2017; 16(2): 62-71.
7. Hosseininezhad M, Ebrahimi H, Seyedsaadat SM, Bakhshayesh B, Asadi M, Ghayeghran AR. Awareness toward stroke in a population-based sample of Iranian adults. Iran J Neurol 2017; 16(1): 7-14.
8. Humphries SE, Morgan L. Genetic risk factors for stroke and carotid atherosclerosis: insights into pathophysiology from candidate gene approaches. Lancet Neurol 2004; 3(4): 227-35.
9. Fan Y, Chen H, Li A, Shi Y, Zhang Y, Feng Q, et al. A promoter polymorphism (rs17222919, -1316T/G) of ALOX5AP gene is associated with decreased risk of ischemic stroke in two independent Chinese populations. PLoS One 2015; 10(3): e0122393.
10. Wang Y, Wang GN, Sun H, Chen C, Xiao H, Zhang JS. Association of ALOX5AP with ischemic stroke in eastern Chinese. World J Emerg Med 2012; 3(2): 108-13.
11. Yang D, Huang X, Cui C, Zhang Y, Li Y, Zang X, et al. genetic variants in the transcriptional regulatory region of the alox5ap gene and susceptibility to ischemic stroke in chinese populations. Sci Rep 2016; 6: 29513.
12. Zargari P, Ghasemi MR, Pirhoushiaran M, Vakili V, Hami J, Farzadfard MT, et al. Renin-angiotensin a1166c polymorphism and the risk of stroke. Journal of Cell and Molecular Research 2015; 7 (1): 32-7.
13. Kim DH, Ahn WY, Kim DK, Choe BK, Kim SK, Jo DJ, et al. A promoter polymorphism (rs17222919, -1316T/G) of ALOX5AP is associated with intracerebral hemorrhage in Korean population. Prostaglandins Leukot Essent Fatty Acids 2011; 85(3-4): 115-20.
Published
2019-06-16
How to Cite
1.
Erfani M, Sadr-Nabavi A, Jafarzadeh-Esfehani R, Shariati M, Ghanbari-Garekani L, Vojdani-Chahchaheh S, Sasannejad P. Association of 5-lipoxygenase activating protein gene polymorphism and stroke: A study from north east of Iran. Iran J Neurol. 18(3):114-118.
Section
Original Article(s)